Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Tom Haltern
    Junior Member
    • Feb 2010
    • 7

    library preparation cost

    Dear all, I'm trying to get a view of a full economics cost for a library prep for the Illumina GA. I certainly know that there is different commercial kits out there but I was wondering what you estimate/calculate for additional purification steps (not included in the kits), for size selection and for overhead? Thanks. Tom
  • peromhc
    Senior Member
    • Sep 2009
    • 108

    #2
    what type of library do you want? DNA, mRNA, etc...

    Comment

    • Tom Haltern
      Junior Member
      • Feb 2010
      • 7

      #3
      I'm talking fragmented DNA only. No CHIP or RNA....

      Comment

      • peromhc
        Senior Member
        • Sep 2009
        • 108

        #4
        it is about $225 per sample for >10 preps, using NEB kit and a few bioanalyzer runs, plus adapters/primers from IDT or wherever..

        Comment

        • upenn_ngs
          Member
          • Sep 2009
          • 70

          #5
          Originally posted by peromhc View Post
          it is about $225 per sample for >10 preps, using NEB kit and a few bioanalyzer runs, plus adapters/primers from IDT or wherever..
          it is possible to develop a sample prep protocol <$65 per sample

          Comment

          • peromhc
            Senior Member
            • Sep 2009
            • 108

            #6
            Originally posted by upenn_ngs View Post
            it is possible to develop a sample prep protocol <$65 per sample
            Using which reagents? At what scale?

            Comment

            • upenn_ngs
              Member
              • Sep 2009
              • 70

              #7
              Originally posted by peromhc View Post
              Using which reagents? At what scale?
              full scale NEBnext enzymes, primers from Operon, agencourt SPRI bead clean-up

              Comment

              Latest Articles

              Collapse

              • SEQadmin2
                Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
                by SEQadmin2


                Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

                The systematic characterization of the human proteome has
                ...
                07-20-2026, 11:48 AM
              • SEQadmin2
                Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
                by SEQadmin2



                Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
                ...
                07-09-2026, 11:10 AM
              • SEQadmin2
                Cancer Drug Resistance: The Lingering Barrier to Rising Survival
                by SEQadmin2



                Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

                There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
                07-08-2026, 05:17 AM

              ad_right_rmr

              Collapse

              News

              Collapse

              Topics Statistics Last Post
              Started by SEQadmin2, 07-24-2026, 12:17 PM
              0 responses
              26 views
              0 reactions
              Last Post SEQadmin2  
              Started by SEQadmin2, 07-23-2026, 11:41 AM
              0 responses
              20 views
              0 reactions
              Last Post SEQadmin2  
              Started by SEQadmin2, 07-20-2026, 11:10 AM
              0 responses
              28 views
              0 reactions
              Last Post SEQadmin2  
              Started by SEQadmin2, 07-13-2026, 10:26 AM
              0 responses
              38 views
              0 reactions
              Last Post SEQadmin2  
              Working...