Hi all,
I'm so pleased to be part of the community and I hope you can help me. Our company have just purchased a Miseq analyser and I need to get a handle on how it works and the protocols for setting up mRNA sequencing analysis, could you please point me in the direction. I should be looking to understand about sample prep and isolation of mRNA from total RNA. mRNA integrity analysis and anything else you may find helpful to get me started. I hope you wont mind me asking stupid questions (I'm sure there will be a lot) until I get a hang of what's going on. I think were also looking at getting an Agilant 2100 analyser too, so anything on that would be good as well. So anything on pitfalls or recommended equipment would be a huge help.
Many thanks
Brian
I'm so pleased to be part of the community and I hope you can help me. Our company have just purchased a Miseq analyser and I need to get a handle on how it works and the protocols for setting up mRNA sequencing analysis, could you please point me in the direction. I should be looking to understand about sample prep and isolation of mRNA from total RNA. mRNA integrity analysis and anything else you may find helpful to get me started. I hope you wont mind me asking stupid questions (I'm sure there will be a lot) until I get a hang of what's going on. I think were also looking at getting an Agilant 2100 analyser too, so anything on that would be good as well. So anything on pitfalls or recommended equipment would be a huge help.
Many thanks
Brian
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