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  • gao gao
    Junior Member
    • Dec 2024
    • 3
    #1

    "single cell" "SMART-seq3" "cDNA library" "sharp peak" and “byproduct”

    Hi，All
    My question is that during the SMART-seq3 testing for single-cell RNA sequencing, after reverse transcription and PCR, the products either show wide peaks of byproducts, or in the 1000-2000 bp range, I am unable to obtain a smooth peak, as there are always many sharp peaks. Has anyone attempted to eliminate these?

    just like B AND C

    Click image for larger version Name: image.png Views: 277 Size: 106.4 KB ID: 326343Click image for larger version Name: image.png Views: 231 Size: 101.1 KB ID: 326344​​
    thanks
    gao gao
    24.12.25
    Tags: sharp peak, single cell rna seq, smart-seq3
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