Hi all,
This is my first time making library for ChIP-seq. I was using trueseq v2 lt sample prep kit. I did the size selection after ligation and cut the gel between 250bp and 300bp. But after PCR amplification, the library size was increased to more than 350 bp
. Any one has similar issue? Thanks for any advise for trouble shooting.
This is my first time making library for ChIP-seq. I was using trueseq v2 lt sample prep kit. I did the size selection after ligation and cut the gel between 250bp and 300bp. But after PCR amplification, the library size was increased to more than 350 bp
. Any one has similar issue? Thanks for any advise for trouble shooting.
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