size shift after PCR amplification

zsf8802616

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#1

size shift after PCR amplification

04-04-2014, 01:20 PM

Hi all,
This is my first time making library for ChIP-seq. I was using trueseq v2 lt sample prep kit. I did the size selection after ligation and cut the gel between 250bp and 300bp. But after PCR amplification, the library size was increased to more than 350 bp . Any one has similar issue? Thanks for any advise for trouble shooting.
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EmiVig

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#2

11-11-2014, 03:03 AM

Hi,

I have exactly the same problem...did you solve it? Could you please update me?! It would be incredibly helpful! I´m really getting crazy with this ChIP library prep..
THANKS A LOT!
Emilia
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