I recently did a RNA extraction using Trizol, I followed all the steps, except I skipped the optional step. I intend to do a RNA seq with these samples using the miseq. The trouble I'm running into are the RIN numbers i get from the bioanalyzer are really bad across samples. The samples are pretty fresh (less than 3 weeks old), were immediately frozen on dry ice, and then kept in a -80 till extraction. Extractions were then also done on ice.
I also nano dropped these samples and the 280/260 values are decent(>1.8), however, the 260/230 are very low, always less than 1.
I was wondering if anyone can help interpret some of my traces and offer any advice as to how to fix these samples so that they are still useable.
I attached the traces.
Thanks,
Mike
I also nano dropped these samples and the 280/260 values are decent(>1.8), however, the 260/230 are very low, always less than 1.
I was wondering if anyone can help interpret some of my traces and offer any advice as to how to fix these samples so that they are still useable.
I attached the traces.
Thanks,
Mike
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