NEBNext Ultra-Directional RNA Library Prep Kit

[anna_m]

Hello,
Has anyone tried the NEBNext Ultra Directional RNA Library Prep Kit? If so, how were your final libraries? Any feedback on this would be great, thanks.

https://www.neb.com/products/e7420-n...t-for-illumina

All the best,
Anna.

[egalvez]

Hi Anna a little bit late the reply,
I am using the protocol and it's really good when you have a good RNA amount, I used this kit for gut-microbiota in combination with Ribozero and I got consistent results, I have to say that I do not have experience with others, but in comparison with an in-home ligation protocol this NEB performs well …it’s fast and isn’t so expensive.

Best and good luck

[aubrie]

Does anyone have experience with automating this kit on the EpMotion?

Thank you,
Aubrie