captainentropy,
Thanks for your reply!
Actually I'm at the step before library construction. I figure I have to have decent amount of ChIP enriched DNA before I start doing library construction.
'half of the peaks are real' means, for example, I got 300 peaks from peak calling software (MACS/Cisgenome, etc). I manually checked these peaks and compared with other lab members' similar result and only about half of these peaks seem real. The other peaks are just background peaks that also exist in a control sample, or are too short to be real.
Hope this is clear?
thanks.
-Sherry
Thanks for your reply!
Actually I'm at the step before library construction. I figure I have to have decent amount of ChIP enriched DNA before I start doing library construction.
'half of the peaks are real' means, for example, I got 300 peaks from peak calling software (MACS/Cisgenome, etc). I manually checked these peaks and compared with other lab members' similar result and only about half of these peaks seem real. The other peaks are just background peaks that also exist in a control sample, or are too short to be real.
Hope this is clear?
thanks.
-Sherry
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