Hello, I'm preparing some RNA for RNAseq and I submitted my samples to the local sequencing core for quality control on the bioanalyzer. The results came back and my samples have good RIN values, almost all >9. But I can't see a nice bump for the mRNA in which I am interested. I'm not sure if all of the peaks along the electrophoretogram are obscuring the mRNA. Should I trust these samples based on the RIN value alone? I want to proceed to ribosome depletion but I don't want to waste an expensive kit on samples that might not have good mRNA in them.
The attached is an example of my bioanalyzer results.
Thanks!
The attached is an example of my bioanalyzer results.
Thanks!
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