Tweet
Has anyone used TruSeq RNA or NEBNext RNA kits and tried to scale the volumes down or up? Is this even possible? Just curious to see if I can add more to generate more libraries, or less to proportionally add less RNA input.
-
-
Scaling up a reaction volume will not result in more library, unless you scale up the input accordingly. Pretty much all NGS kits should already get you much more library than you need for sequencing if you use the highest recommended input, so there wouldn't be much point in scaling up a reaction, in my opinion.
Scaling down a reaction could be performed, but it probably will not allow you to go below the recommended input. The biggest advantage of scaling down is saving money. Scaling down may require some slight optimization, but if you have a lot of samples to prep it may be worth the optimization if you can make a kit last twice as long.
-
During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.
Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...09-09-2024, 10:59 AM -
The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...08-27-2024, 04:44 AM
Comment