What are people using as best practice for plasticware in NGS library preparation? For 1.5 and 0.6 ml tubes, I've been using Eppenforf's LoBind tubes (library storage and dilution). I primarily use strip tubes for library generation, but I haven't found any 0.2mL tubes that are meant to prevent DNA sticking. I wonder if anybody has any input on what plastic is best for low-concentration RNA/DNA, and if anyone has any specific evidence to support it. Thanks!
-
-
Hi,
I had the same problem and I searched for a long time some low-binding 0.2 ml tubes or plates without success. Then I found a very old paper (attached) where they showed that, when adding some detergent to the solutions/mixes, the adsorption of DNA to the tube was negligible. We are doing single-cell RNA-seq and we have 0.1% Triton in the lysis buffer. We manage to achieve a better sensitivity than the commercial kits, therefore I believe that having some detergent is enough for our purpose and we don´t need low-binding tubes.
I hope that this helps!
Best,
SimoneLast edited by Simone78; 08-24-2017, 05:42 AM. -
Hey there, I know this is an old thread, but what is the citation for the "very old paper" you mention in your reply to clint ?Comment
-
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment