What are people using as best practice for plasticware in NGS library preparation? For 1.5 and 0.6 ml tubes, I've been using Eppenforf's LoBind tubes (library storage and dilution). I primarily use strip tubes for library generation, but I haven't found any 0.2mL tubes that are meant to prevent DNA sticking. I wonder if anybody has any input on what plastic is best for low-concentration RNA/DNA, and if anyone has any specific evidence to support it. Thanks!
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Hi,
I had the same problem and I searched for a long time some low-binding 0.2 ml tubes or plates without success. Then I found a very old paper (attached) where they showed that, when adding some detergent to the solutions/mixes, the adsorption of DNA to the tube was negligible. We are doing single-cell RNA-seq and we have 0.1% Triton in the lysis buffer. We manage to achieve a better sensitivity than the commercial kits, therefore I believe that having some detergent is enough for our purpose and we don´t need low-binding tubes.
I hope that this helps!
Best,
SimoneLast edited by Simone78; 08-24-2017, 05:42 AM. -
Hey there, I know this is an old thread, but what is the citation for the "very old paper" you mention in your reply to clint ?Comment
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The immune system’s power comes from its genetic diversity, allowing myriad threats to be neutralized through first recognizing foreign antigens. That diversity is also what makes the immune system so difficult to study. Recent advances in sequencing technology and computational biology, however, are giving researchers new tools to understand immune responses and immune-related diseases in greater detail.
This convergence of genetics, immunology, and computation...Yesterday, 05:41 AM
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