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What are people using as best practice for plasticware in NGS library preparation? For 1.5 and 0.6 ml tubes, I've been using Eppenforf's LoBind tubes (library storage and dilution). I primarily use strip tubes for library generation, but I haven't found any 0.2mL tubes that are meant to prevent DNA sticking. I wonder if anybody has any input on what plastic is best for low-concentration RNA/DNA, and if anyone has any specific evidence to support it. Thanks!
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Hi,
I had the same problem and I searched for a long time some low-binding 0.2 ml tubes or plates without success. Then I found a very old paper (attached) where they showed that, when adding some detergent to the solutions/mixes, the adsorption of DNA to the tube was negligible. We are doing single-cell RNA-seq and we have 0.1% Triton in the lysis buffer. We manage to achieve a better sensitivity than the commercial kits, therefore I believe that having some detergent is enough for our purpose and we don´t need low-binding tubes.
I hope that this helps!
Best,
SimoneLast edited by Simone78; 08-24-2017, 05:42 AM. -
Hey there, I know this is an old thread, but what is the citation for the "very old paper" you mention in your reply to clint ?Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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