We are trying to adapt the ampure xp protocol into an automated library prep system but we're having a few problems over the volumes. We are using NEBNext fragmentase kits to fragment the DNA and the NEBNext DNA sample prep mastermix set 1 to make our libraries, any advice would be gratefully appreciated - Thanks...
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM