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  • Ancestro
    Member
    • Oct 2015
    • 15
    #1

    Ampure XP DNA rebinding if accidentaly added RSB

    Hi to all!

    I was preparing a library, but by accident (my mind was on other place i think) i added the RSB (Resuspension Buffer from Truseq RNa library prep kit) after the EtOH 80% wash, but the ethanol was not completely dried...

    Did I messed up this library? Cause the RSB elutes the DNA from the beads. Is there a way to recover the DNA, or just Im a little paranoic right now and my DNA will still be fine?
    Tags: None
    • nucacidhunter
      Jafar Jabbari
      • Jan 2013
      • 1250
      #2
      If you have collected RSB from beads you can dilute the eluate to reduce Ethanol concentration and do a column or another bead clean up.

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