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Hi,
How to select an optimal set of indexes from different library prep kits to design a sequence run?
An example: I am about to prepare libraries and sequence 40 samples prepared with XT dual indexing and 21 libraries prepared with single indexes (ScriptSeq).
Given a set of 8 i7 indexes from the XT libraries the task is to select a set 21 index among 48 available that will differ on at least two positions relative to the 8 i7 indexes.
It is quite tedious and error-prone to do this manually.
Does anyone have a piece of script to do this?
Or how do you select an optimal set of indexes?
Cheers,
/Jakob
How to select an optimal set of indexes from different library prep kits to design a sequence run?
An example: I am about to prepare libraries and sequence 40 samples prepared with XT dual indexing and 21 libraries prepared with single indexes (ScriptSeq).
Given a set of 8 i7 indexes from the XT libraries the task is to select a set 21 index among 48 available that will differ on at least two positions relative to the 8 i7 indexes.
It is quite tedious and error-prone to do this manually.
Does anyone have a piece of script to do this?
Or how do you select an optimal set of indexes?
Cheers,
/Jakob
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