Hi all,
I am looking for a bead based library normalization method as an alternative to the library normalization in the NexteraXT kit.
On the web, I have found commercial kits like the Mag-Bind® EquiPure Library Normalization Kit (Omega Bio-Tek), ALine DNA Normalizer (96 well) v3 (Aline Biosciences or the MagQuant ™ DNA Kit (MagBioGenomics)
I have also found a sort of homebrew protocol, based on AMPure beads (BeNUS protocol; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133082/)
Does anyone have experiences with one or more of the above methods, or has an alternative method?
I would like to avoid QPCR measurements as it is the idea to easily upscale/automate the normalization.
Thank you in advance!
Wim
I am looking for a bead based library normalization method as an alternative to the library normalization in the NexteraXT kit.
On the web, I have found commercial kits like the Mag-Bind® EquiPure Library Normalization Kit (Omega Bio-Tek), ALine DNA Normalizer (96 well) v3 (Aline Biosciences or the MagQuant ™ DNA Kit (MagBioGenomics)
I have also found a sort of homebrew protocol, based on AMPure beads (BeNUS protocol; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4133082/)
Does anyone have experiences with one or more of the above methods, or has an alternative method?
I would like to avoid QPCR measurements as it is the idea to easily upscale/automate the normalization.
Thank you in advance!
Wim
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