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Are there any new alternatives out there for size selection other than the Pippin prep? I saw some older posts on the LabChip XT but don't know if that is even manufactured at this point. I also saw some on the E-gel; it didn't look like it was useful for libraries. Information on any recent worthy alternatives that should be considered would be appreciated (100 - 750 bp range).
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SageElf
Also, has anyone used the SageElf? It fractionates the entire DNA smear into separate tubes by rotating the electrical field 90 degrees. -
Coastal Genomics has a very high throughput instrument.Comment
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We will also be showcasing a lower-throughput option that can integrate with robotic platforms or be used by a technician as SLAS in Washington DC. Please message me if you would like more details.Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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