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  • krutipedali
    Junior Member
    • Nov 2016
    • 3
    #1

    On-Plate ATAC-seq

    Hello,

    Could anyone please drop any comments on libraries that i have prepared using on-plate ATAC protocol (DOI: 10.1186/s13059-015-0840-9). Libraries seem to be overtagmented, but i would like to receive any comment considering their sequencibility and further actions of optimising tagmentation protocol. This time i used standard NEXTERA protocol(2,5ul of enzyme) and 50000 HeLa cells.

    Thanks in advance.
    Attached Files
    Tags: atac-seq, library preparation
  • Dampor
    Member
    • Jun 2012
    • 14
    #2
    Hi,
    did you get any feedback on those libraries?
    I have got something similar but from different cells.
    Did you sequenced them at the end? if yes, how was the result?

    Thanks
    Dam

    Comment

    • krutipedali
      Junior Member
      • Nov 2016
      • 3
      #3
      No, those libraries haven`t been sent for sequencing. Instead we have produced another samples. The thing is they are the way too overtagmented, so go down with incubation time(even to 5-10 minutes) or try removing permeabilization step.

      Comment

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