We have prepared ATAC libraries for 24 samples following the protocol from Buenrostro et.al, using their custom Nextera-like primers and we have attempted to sequence these on NextSeq.
We're essentially getting no reads and can't figure out why. The libraries show good fragment distribution on Tapestation, and we have also run a library quantification qPCR which also shows that there is sufficient sequenceable material.
We're completely stuck, and Illumina as well as the ATAC forum have been of little help...
If anyone can help, it would be very much appreciated!
We're essentially getting no reads and can't figure out why. The libraries show good fragment distribution on Tapestation, and we have also run a library quantification qPCR which also shows that there is sufficient sequenceable material.
We're completely stuck, and Illumina as well as the ATAC forum have been of little help...
If anyone can help, it would be very much appreciated!
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