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  • Dorota
    Junior Member
    • May 2017
    • 9
    #1

    Overloading QiaAmp minelute virus spin columns

    Hi,
    did anyone try to include more volume than recommended 200uL on QiaAmp minelute virus spin kit columns ? As for exapmle just loading the lysed sample in two rounds on the column to bind? I want to load 400uL, human cells.
    Any tips/suggestions? Did it have very negative impact on your yield?

    (I know there are different kits, also QiaAmp vacuum, but I would really like to use the one mentioned above)
    Tags: None
    • Dorota
      Junior Member
      • May 2017
      • 9
      #2
      If anyone wonders, it kind of works :]

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