Illumina is going to re-introduce ribozero equivalent kits in 2020.
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For those seeking a bacterial rRNA depletion kit, NEB has just released a kit that works very well in our hands.
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DISCLAIMER: I work for ZymoOriginally posted by cement_head View Post
We have options for people who are interested in doing probe-free depletion. Contact [email protected] and tell them you'd like to try the RiboFree Total RNA Library Kit. We were offering trial sizes and free samples a few months ago.Comment
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According to rumors, RiboZero will make a comeback. Not exactly the same products though; it will use a new chemistry.Comment
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Illumina did release a new version of RiboZero+ yesterday: https://www.illumina.com/products/by...depletion.htmlComment
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Interesting. Appears to use a strand-denaturation/hybridization-based normalization to remove/reduce concentrations of highly represented cDNAs. That is, enzymatic digestion of DNA/RNA duplexes that form due to C0t kinetics.Originally posted by jjlin View Post
This would make it species agnostic. Specified to work down to 100ng or somewhat below that with extended hybridization times.
Most of the charm of the old Truseq Ribozero-depletion library kits was that although they specified a limited range of species, eg "human/mouse/rats" the probes were very long (not oligos) and so actually seemed to work on any animal. For example they worked on moth RNA in our hands.
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PhillipComment
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Thanks. No manual available yet. List price is $82 per reaction.
Originally posted by GenoMax View PostComment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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