Just annealing two single strand DNA? Is there any following purification steps needed? like ethanol precipitation?
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No there is no need for further treatment. Two very good descriptions of the process are the Illumina patent ((WO/2008/015396) METHOD OF LIBRARY PREPARATION AVOIDING THE FORMATION OF ADAPTOR DIMERS)) and the supplementary methods for the paper Identification of genetic variants using bar-coded multiplexed sequencing Nat Methods.2008 Oct;5(10):887-*‐93.Originally posted by chromatin View Post
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