Just annealing two single strand DNA? Is there any following purification steps needed? like ethanol precipitation?
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No there is no need for further treatment. Two very good descriptions of the process are the Illumina patent ((WO/2008/015396) METHOD OF LIBRARY PREPARATION AVOIDING THE FORMATION OF ADAPTOR DIMERS)) and the supplementary methods for the paper Identification of genetic variants using bar-coded multiplexed sequencing Nat Methods.2008 Oct;5(10):887-*‐93.Originally posted by chromatin View Post
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Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
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