Tweet
I am preparing RNA libraries using the TruSeq Total RNA kit from Illumina and I am trying to use NextFlex adapters in lieu of the truseq ones. I am stuck on the correct concentration to use and Illumina tells me the molarity of their adapters is proprietary. Does anyone know the correct concentration of their adapters, I don't have any on hand to test.
-
-
Check the concentration of the Nextflex RNA-seq adapters on their web page; I have not memorized it but it will work. -
Thanks Luc, I know the concentration of the nextflex adapters and I know they will work. I am looking for the concentration of the illumina adaptersComment
-
Nextflex adapters are available for DNA applications and for RNA-seq applications. The latter are merely dilutions of the former, but Bioo provides the concentration info somewhere.
If you have the diluted Nextflex adapters (for RNA-seq) use the same adapter volume as in the TruSeq RNA kit.Comment
-
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...06-18-2026, 07:11 AM -
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
...06-02-2026, 10:05 AM -
With the launch of new single-cell sequencing platforms in 2026, the field stands at an exciting inflection point. This article surveys the most impactful advances in the field and discusses how they’re reshaping research in cancer, immunology, and beyond.
Introduction
Single-cell sequencing technologies have undergone remarkable advances over the past decade, transitioning from low-throughput experimental approaches to highly scalable platforms capable of...05-22-2026, 06:42 AM
Comment