I am preparing RNA libraries using the TruSeq Total RNA kit from Illumina and I am trying to use NextFlex adapters in lieu of the truseq ones. I am stuck on the correct concentration to use and Illumina tells me the molarity of their adapters is proprietary. Does anyone know the correct concentration of their adapters, I don't have any on hand to test.
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Check the concentration of the Nextflex RNA-seq adapters on their web page; I have not memorized it but it will work. -
Thanks Luc, I know the concentration of the nextflex adapters and I know they will work. I am looking for the concentration of the illumina adaptersComment
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Nextflex adapters are available for DNA applications and for RNA-seq applications. The latter are merely dilutions of the former, but Bioo provides the concentration info somewhere.
If you have the diluted Nextflex adapters (for RNA-seq) use the same adapter volume as in the TruSeq RNA kit.Comment
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM
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