Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • seqsuave
    Junior Member
    • May 2018
    • 9

    #1

    AmpliSeq vs TruSeq

    Now that Illumina sells AmpliSeq, I am trying understanding the difference between AmpliSeq for Illumina and TruSeq Targeted panels. From what I can tell AmpliSeq can probe for more targets, and deal with lower input and lower sample quality.
    Are there any other big differences I am missing between those libraries prep methods?

    Also, what experience or opinions does anyone have when comparing these two kits?
    Last edited by seqsuave; 03-14-2019, 12:44 PM.
  • labking
    Junior Member
    • Jun 2019
    • 1

    #2
    So, we had to change from TSCA to AmpliSeq. My experience about AmpliSeq right now that this is not as good as TSCA. There is not advantage regarding library preperation, it is just different. How to analyze the AmpliSeq data was difficult since I needed the Tech Support. A manual where everything is written down would be helpful.
    First of all, the in silico coverage is lower when designing a new panel with exactly the same content as for TSCA. For TSCA we had a 100% coverage and for AmpliSeq just around 98%. We would require concierge to fill this gap but this would take another three months. Secondly, the higher the number of amplicons the lower the in silico coverage. Well, this is normal but way worse than for TSCA. Finally, we ordered a panel where the in silico coverage was a 100% but one amplicon dropped out. This was not worse as for TSCA.
    I would like to knoe the experience by other users. Or is it us?

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Beyond CRISPR/Cas9: Understand, Choose, and Use the Right Genome Editing Tool
      by SEQadmin2



      CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).

      Despite this, “CRISPR helped turn genome editing from a specialized technique into
      ...
      07-31-2026, 11:01 AM
    • SEQadmin2
      Proteomic Platforms: How to Choose the Right Analytical Strategy to Improve Detection and Clinical Applications
      by SEQadmin2


      Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.

      The systematic characterization of the human proteome has
      ...
      07-20-2026, 11:48 AM
    • SEQadmin2
      Advanced Sequencing Platforms Tackle Neuroscience’s Toughest Genomics Problems
      by SEQadmin2



      Genomics studies in neuroscience face a special challenge due to the brain’s complexity and scarcity of samples. Mapping changes in cell type and state using conventional next-generation sequencing methods remains challenging. Advances in technologies like single-cell sequencing, spatial transcriptomics, and long-read sequencing have opened the door to deeper studies of the brain and diseases like Alzheimer’s, amyotrophic lateral sclerosis (ALS), and schizophrenia.
      ...
      07-09-2026, 11:10 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 08-03-2026, 10:13 AM
    0 responses
    15 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-31-2026, 02:55 AM
    0 responses
    32 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-24-2026, 12:17 PM
    0 responses
    23 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-23-2026, 11:41 AM
    0 responses
    21 views
    0 reactions
    Last Post SEQadmin2  
    Working...