Got any sequencing wizards out there? Undergrad barcoded 10x 96 well 16S amplicon plates for a miSeq muliplex run with the same set of Nextera barcodes for all the plates. Ideas other than starting over?
I'm wondering if I can ligate another barcode with a different adapter sequence for a hiSeq (like UD) so each plate has a unique barcode then each well would have an its x,y combination.
I'm wondering if I can ligate another barcode with a different adapter sequence for a hiSeq (like UD) so each plate has a unique barcode then each well would have an its x,y combination.
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