Hi there,
I am interested in performing polysome profiling with downstream application for RNAseq of monosome and polysome-associated RNAs.
Has anyone had much experience with choice of library preparations for monosome and polysome-associated RNAs?
ribosomal RNA surely is to be more abundant in these samples compared to total RNA extractions? This would lead me towards preferring ribosome-depletion (e.g. riboZero) as opposed to polyA. However PolyA is better for intact fragments and we also intend to sequence total RNA where polyA would be preferred. If we want to compare sequencing between total RNA and mono/poly RNAs it would make sense to use the same library prep?
If you have any experience in choice of library prep (poly A vs ribo-depletion) for polysome-profiling RNAseq experiments please let me know which method you preferred and why
Many thanks,
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
Latest Articles
Collapse
-
by seqadmin
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...-
Channel: Articles
04-22-2024, 07:01 AM -
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, Today, 11:49 AM
|
0 responses
12 views
0 likes
|
Last Post
by seqadmin
Today, 11:49 AM
|
||
Started by seqadmin, Yesterday, 08:47 AM
|
0 responses
16 views
0 likes
|
Last Post
by seqadmin
Yesterday, 08:47 AM
|
||
Started by seqadmin, 04-11-2024, 12:08 PM
|
0 responses
61 views
0 likes
|
Last Post
by seqadmin
04-11-2024, 12:08 PM
|
||
Started by seqadmin, 04-10-2024, 10:19 PM
|
0 responses
60 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 10:19 PM
|