Rapid Library Adapter Dimer

[Br3ndan]

Hi All,

We have recently done a 454 GS junior run of a rapid library build of some low-copy number aDNA and had an overwhelming number of reads appearing to be dimerisation of our y-adapters. Has anyone else experienced this?

Thanks

[lalremruata]

What i did was made aDNA library using RAPID lib Kit. Amplified with emPCR primer and then cloned the amplified library to ensure fragment-adaptor ligation. of course my purpose was to enrich using NimbleGen array prior to 454 GS junior sequencing!!