Hello,
I have been trying to extract chromatin from an immune system population, but the DNA concentration levels that I've been getting are extremely underwhelming.
Namely, from 1 million to 3 million cells I am getting concentrations of around 0.525ng/uL to 2.08ng/uL of chromatin in 50uL of SDS lysis buffer (1%SDS, 100mM EDTA, 50mM Tris pH 8.0), measured on Qubit with the dsDNA HS kit.
Fixation was done with Formaldehyde 37% for 10 minutes.
Anyone with experience on extracting chromatin from immune cells? I need to know if these are normal concentration values for these cells, because I am at a loss already.
I have been trying to extract chromatin from an immune system population, but the DNA concentration levels that I've been getting are extremely underwhelming.
Namely, from 1 million to 3 million cells I am getting concentrations of around 0.525ng/uL to 2.08ng/uL of chromatin in 50uL of SDS lysis buffer (1%SDS, 100mM EDTA, 50mM Tris pH 8.0), measured on Qubit with the dsDNA HS kit.
Fixation was done with Formaldehyde 37% for 10 minutes.
Anyone with experience on extracting chromatin from immune cells? I need to know if these are normal concentration values for these cells, because I am at a loss already.
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