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Hello,
The Illumina protocol says to use Klenow exo- along with dATP to add 3' A base to the ends of your blunt ended DNA, essentially extending your blunt ended DNA a base (extension step). Then the adapters with T base on one end will ligate easily to the A in the presence of DNA ligase.
What happens when too much Klenow exo- is added to the extension step? And what happens during the ligation if excess Klenow exo- is added? Anyone know?
The Illumina protocol says to use Klenow exo- along with dATP to add 3' A base to the ends of your blunt ended DNA, essentially extending your blunt ended DNA a base (extension step). Then the adapters with T base on one end will ligate easily to the A in the presence of DNA ligase.
What happens when too much Klenow exo- is added to the extension step? And what happens during the ligation if excess Klenow exo- is added? Anyone know?
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