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  • seqgirl123
    Member
    • Oct 2008
    • 78
    #1

    need help with klenow

    Hello,
    The Illumina protocol says to use Klenow exo- along with dATP to add 3' A base to the ends of your blunt ended DNA, essentially extending your blunt ended DNA a base (extension step). Then the adapters with T base on one end will ligate easily to the A in the presence of DNA ligase.

    What happens when too much Klenow exo- is added to the extension step? And what happens during the ligation if excess Klenow exo- is added? Anyone know?
    Tags: None
  • ECO
    --Site Admin--
    • Oct 2007
    • 1360
    #2
    Hey seqgirl, please don't cross post the exact same question. I deleted the one in the Illumina forum, I think it belongs here.

    Thanks!

    Comment

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