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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by vl80, 06-08-2014, 11:06 PM
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1 response
5,167 views
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Started by Ayeh, 06-07-2014, 09:18 AM
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1,097 views
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by Ayeh
06-07-2014, 09:18 AM
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Started by skerker, 06-05-2014, 05:56 PM
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1,675 views
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by skerker
06-05-2014, 05:56 PM
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Started by Darkside, 05-09-2014, 08:43 AM
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3 responses
2,117 views
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by lyndaben
06-05-2014, 01:46 PM
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Started by brynmcln, 06-03-2014, 07:56 PM
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3 responses
3,004 views
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by lorendarith
06-05-2014, 06:32 AM
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Started by TonyBrooks, 05-30-2014, 07:55 AM
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3 responses
2,139 views
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by TonyBrooks
06-02-2014, 02:37 AM
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Started by cordsp, 05-28-2014, 09:46 AM
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0 responses
1,473 views
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by cordsp
05-28-2014, 09:46 AM
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Started by zwang0614, 05-28-2014, 12:33 AM
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3 responses
1,532 views
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Started by snarapor, 05-26-2014, 02:58 AM
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5 responses
5,688 views
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Started by Katerina Cvikova, 05-27-2014, 12:18 AM
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1 response
1,478 views
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by Etherella
05-27-2014, 01:02 AM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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Started by SEQadmin2, Yesterday, 10:08 AM
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by SEQadmin2
Yesterday, 10:08 AM
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Started by SEQadmin2, 07-07-2026, 11:05 AM
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07-07-2026, 11:05 AM
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Started by SEQadmin2, 07-02-2026, 11:08 AM
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07-02-2026, 11:08 AM
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