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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by twang11, 02-22-2012, 05:18 PM
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by twang11
02-22-2012, 05:18 PM
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Started by OptimusBrien, 12-15-2010, 09:12 AM
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7 responses
13,150 views
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by xiaoyuan zi
02-22-2012, 01:18 PM
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Started by sehrrot, 02-12-2012, 09:24 PM
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1 response
2,787 views
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by Rocketknight
02-22-2012, 05:15 AM
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Started by Juergen F, 02-21-2012, 10:09 AM
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1,695 views
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by Juergen F
02-21-2012, 10:09 AM
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Started by ulz_peter, 02-02-2012, 03:38 AM
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2 responses
2,544 views
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by ECO
02-20-2012, 11:57 AM
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Started by Coyk, 02-15-2012, 07:50 AM
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1 response
3,424 views
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by pmiguel
02-18-2012, 07:49 PM
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Started by joytang1028, 02-17-2012, 03:37 PM
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2,271 views
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by joytang1028
02-17-2012, 03:37 PM
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Started by moedog45, 02-15-2012, 05:50 PM
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1 response
2,418 views
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by liliniu
02-16-2012, 10:08 AM
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Started by oryctolagus, 02-15-2012, 03:33 AM
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1 response
2,647 views
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by TonyBrooks
02-15-2012, 05:18 AM
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Started by Staph, 03-18-2011, 12:38 AM
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8 responses
5,436 views
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Last Post
by Heisman
02-15-2012, 05:07 AM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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