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Techniques and protocol discussions on sample preparation, library generation, methods and ideas
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Started by kvanderlinden, 08-03-2018, 03:13 PM
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Started by Buckethead84, 07-30-2018, 08:36 AM
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1 response
2,661 views
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by HESmith
07-30-2018, 04:43 PM
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Started by emily202, 07-12-2018, 11:25 AM
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6 responses
4,338 views
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by emily202
07-17-2018, 05:55 AM
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Started by StephanK, 07-17-2018, 05:12 AM
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1,624 views
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by StephanK
07-17-2018, 05:12 AM
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Started by seqtechno1, 07-10-2018, 08:12 AM
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2 responses
2,308 views
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by seqtechno1
07-11-2018, 07:04 AM
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Started by viverum, 06-24-2018, 03:08 AM
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3 responses
3,441 views
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by viverum
07-09-2018, 01:03 AM
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Started by _chiara_, 06-15-2018, 01:14 AM
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5 responses
2,984 views
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by JBKri
07-04-2018, 01:00 AM
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Started by davalbuq, 06-15-2018, 05:31 AM
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8 responses
3,094 views
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by davalbuq
07-02-2018, 03:16 AM
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Started by dpryan, 06-21-2018, 05:50 AM
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0 responses
1,364 views
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by dpryan
06-21-2018, 05:50 AM
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Started by SuzuBell, 06-18-2018, 01:31 PM
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1 response
1,312 views
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Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
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