My htseq-count script stops because input is too big (5gb), are there ways to overcome this?
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What input? I cannot imagine that you have a GTF file with 5 GB size. Are you sure you removed any FASTA lines that may have been appended to it?
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htseq-count reads the SAM file line by line and hence, it should not care how big it is, because it only looks at one line at a time. Please post a transcript of your session, with the full error message.
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