My htseq-count script stops because input is too big (5gb), are there ways to overcome this?
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What input? I cannot imagine that you have a GTF file with 5 GB size. Are you sure you removed any FASTA lines that may have been appended to it? -
My input sam-file is to big.Comment
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htseq-count reads the SAM file line by line and hence, it should not care how big it is, because it only looks at one line at a time. Please post a transcript of your session, with the full error message.Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification,...10-30-2026, 06:46 AM
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