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  • arkilis
    Senior Member
    • Jul 2013
    • 119

    #1

    NEWBIE question: does the 5' adapter mean forward primer?

    As in the title.

    3' adapter == reverse primer
    5' adapter == foward primer

    Is that right? Thanks!
  • Kennels
    Senior Member
    • Feb 2011
    • 149

    #2
    No, the adapters are not the primers.
    5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.

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    • arkilis
      Senior Member
      • Jul 2013
      • 119

      #3
      Originally posted by Kennels View Post
      No, the adapters are not the primers.
      5' adapters are what you ligate to your sample sequence (dna, cdna, srna etc) at the 5' end, and 3' adapter at the 3' end. The primers would then anneal to your adapters during PCR amplification.
      thx~~ it is much clear to me now
      Last edited by arkilis; 09-26-2013, 09:24 PM.

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