Hi there,
I have just received my Illumina HiSeq data back. I have 2x250bp runs (so forward and reverse reads).
I ran a FASTQC and found that I have an adaptor sequence present in my data. Should I first remove the adaptors then merge my forward and reverse reads using FLASh? Or should I merge first, then remove the adaptors and trim at the same time (using trimmomatic).
Thank you!
I have just received my Illumina HiSeq data back. I have 2x250bp runs (so forward and reverse reads).
I ran a FASTQC and found that I have an adaptor sequence present in my data. Should I first remove the adaptors then merge my forward and reverse reads using FLASh? Or should I merge first, then remove the adaptors and trim at the same time (using trimmomatic).
Thank you!
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