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Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc

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Started by laura, 05-09-2011, 04:42 AM
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Started by k-gun12, 05-23-2011, 01:10 PM
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Started by sehrrot, 02-21-2011, 05:44 PM
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Started by chenjy, 05-24-2011, 02:47 AM
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Started by john@wurbio, 05-24-2011, 01:27 AM
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Started by ndeshpan, 05-04-2011, 04:29 PM
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Started by jay2008, 05-23-2011, 03:14 PM
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Started by jay2008, 05-23-2011, 03:11 PM
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Started by PFS, 05-20-2011, 11:15 AM
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Started by srividya, 05-21-2011, 12:20 AM
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

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    The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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