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Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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Started by laura, 05-09-2011, 04:42 AM
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35 responses
107,335 views
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by laura
12-05-2013, 02:37 AM
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Started by sci_guy, 01-23-2008, 11:19 PM
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236 responses
683,078 views
1 reaction
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by ECO
12-25-2009, 06:45 PM
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Started by ecSeq Bioinformatics, 06-21-2017, 04:36 AM
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0 responses
3,193 views
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Started by MurielGB, 05-02-2014, 02:03 AM
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14 responses
6,655 views
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by gshieh2
06-21-2017, 02:03 AM
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Started by vandana8, 06-20-2017, 10:21 PM
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0 responses
3,033 views
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by vandana8
06-20-2017, 10:21 PM
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Started by JamesSeward, 06-20-2017, 09:13 AM
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3 responses
1,780 views
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Started by MGCBrown, 06-20-2017, 10:27 AM
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1 response
1,254 views
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by GenoMax
06-20-2017, 10:32 AM
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Started by balaena, 06-20-2017, 06:43 AM
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2 responses
1,505 views
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by balaena
06-20-2017, 08:31 AM
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Started by aprice67, 05-17-2017, 01:13 PM
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7 responses
9,284 views
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by aprice67
06-20-2017, 06:21 AM
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Started by doublealice, 06-18-2017, 07:33 PM
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0 responses
1,554 views
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by doublealice
06-18-2017, 07:33 PM
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Started by kpboh, 06-15-2017, 08:54 AM
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3 responses
1,972 views
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by neavemj
06-18-2017, 03:05 PM
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Started by icanwinwyz, 06-16-2017, 03:34 PM
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1 response
2,079 views
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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