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Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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Started by laura, 05-09-2011, 04:42 AM
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35 responses
107,346 views
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by laura
12-05-2013, 02:37 AM
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Started by sci_guy, 01-23-2008, 11:19 PM
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236 responses
683,150 views
1 reaction
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by ECO
12-25-2009, 06:45 PM
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Started by KevinLam, 03-16-2010, 02:01 AM
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3,537 views
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Started by kushald, 03-11-2013, 11:34 PM
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2 responses
4,244 views
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by bioramg
04-13-2020, 11:57 PM
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Started by jackb123456789, 04-12-2020, 04:02 PM
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0 responses
1,107 views
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Started by Marius, 10-04-2019, 06:51 AM
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1 response
2,845 views
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by KyleeJansen
04-11-2020, 01:44 AM
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Started by polaxgr, 04-08-2020, 03:55 AM
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0 responses
1,376 views
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by polaxgr
04-08-2020, 03:55 AM
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Started by megannj, 04-06-2020, 10:28 AM
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0 responses
1,759 views
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by megannj
04-06-2020, 10:28 AM
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Started by gcR, 03-29-2017, 11:24 AM
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3 responses
3,491 views
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Started by yy273826987, 03-27-2020, 07:34 AM
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1 response
1,083 views
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by yy273826987
04-04-2020, 02:19 PM
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Started by ahodgins, 02-03-2014, 01:00 PM
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1 response
2,892 views
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by DPresto
04-04-2020, 01:55 PM
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Started by GenoMax, 05-01-2015, 08:57 AM
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246 responses
162,076 views
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Last Post
by AndrewP
04-03-2020, 08:40 AM
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by SEQadmin2
Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.
There is no single reason why many patients don’t respond to treatment as expected. Cancer is...-
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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Started by SEQadmin2, 07-07-2026, 11:05 AM
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