What with all the developments in the RNA-Seq library prep field our lab is contemplating moving away from the Illumina Tru-Seq RNA kits and using the Script-Seq kits instead. Between the two the data we have seen is comparable and the time it takes is significantly less. Has anyone done something like this and if so, how has it worked out?
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM