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  • mafireyi
    Junior Member
    • Jul 2015
    • 1

    #1

    Soap denovo input files in contig file from FLASH

    I am using Soap denovo for whole genome assembly. I have use trimmonatic and Flash to trim and merge my reads. Flash spits out 4 output files. My question is which of the output files from Flash should I use in the config file of Soap. Should i include the out.notCombined files together with out.extendedFrags file.

    Thanks
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Solution on Biostars: https://www.biostars.org/p/150182/

    Comment

    • Brian Bushnell
      Super Moderator
      • Jan 2014
      • 2709

      #3
      In my experience, merging reads leads to an inferior Soap assembly, at least for metagenomes. I'm not sure why. I suggest you try both ways (merged and unmerged).

      Comment

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