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Anyone have the reference sequences for the CAT and ATG control beads? I can get the fna for the sequences generated (and would assume most frequent are the correct ones) but would like to be sure. Thanks.
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Got them; short versions (from flowgram "ideal" sequence) and full length versions from gsReporter --analysisParms.parse. Thanks, Teri Rambo Mueller @ Roche for the latter info.
>ECTF301_ATG
ATGCCTAGTAAACAATGTTCGATCCGGCGAAGTCTGCAAGAATCCAGCGCTGCCGGTTCG
TCGGCGCTGTGCCGTGGAGCTGACCTGATCGACGACTCGTGCGATCGAGTATGGCTCGCC
ACCTAGAATCGCGACCTATCATCAACGAAGGCGACATCAATACGTCGCCGCGTTAAATCG
TCACTTTCTGCGCACGTCAGCATTACCGCACAGTGCTGGAGTCACTTCCGTGCCGGTCTC
TGCTGGTGACTTCGCAGACCGTCCTGACGTGCTAGGTGGCCGCTTCGCCTGGCAGCCGAT
GAACGGCGTAGATCGGTGCCCTCGCTGCTAGACTGGCGGTTACGAAATGGACGCGCGCAT
CTACTGTGCGAACGTGCTCGCTACCGGACCTGCCGGTATATGGTGAACACCAACACCTCG
GCAGACCTCTCTGAGCCTAGCAGAGCTTCAACCTGGAAGTTCCGGTGACGATCACGAACG
TATCGACGAAAGTTCAGGAATACGTTGCTACTACATCTAACGCTGACTGGATCGAATACT
CTGACTGCACTTCTGTAGCGCAGCCGTCGTCTAGTCTCCGCCTGTCGTTCCGTTATCAGC
TGACTGAACTTGACGCGCAGCGGGCACGTATCGTACTGTCCGGAAGGTGACGAACGCGTA
CCGTTAAAGCAGCCGTCTATCTGTGCTGAACGTAGGTATCGCAACTTGACGTACTGCTGG
GTAATCCGGCAGAGATCACCGTGTTGCAGCGTCTCAGGGTGTAGAACTGGGTGCAGGGAT
TGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCG
TAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCT
CGGTACGCGCATGACACGCAACAGGGGATAGGGACACGCACGCAACAGATGG
>ECTF302_ATG
ATGCTCGACCGACGGTGTTGTAACGTGACGATTACACTACCTGTCTGCTATCGAACGAAG
GCACTACGTTATCGCCCAGGCGAACTCCAACTTCGGATGAAGAAGGTCCACTTCGTAGAA
GACCTGGTAACTTCGCCGTAGCAAAGGCGTAATCCTAGCTTGTTCAGCCGCGACCAGGTT
GTACTACATGGACGTACTCCACAGACAGGTGGTATCCGTCGGTGACGTCTGATCCCGTCC
TGGAACACGTATGACGACCAACCGTGCATTGATAGGGTGCGAACATGACAACGTCAGGCC
GTTCCGACTCTGACGCGCTGATAACGCCGCTGGTTGGTACTGTATGGAACGTGCTGTTGA
CCGTTGACTCCGGTGTAACTAGCGGTAGCTAAACGTGGTGGTGTCGTTCGAGTACGTGGA
TGCTTCCCGTATCGTTATCGAGTTAACGAAGACGAGATGTATCCGGGTGAAGTCAGGTAT
CGACATCTACAACTGACCAAATACACGTTCTAACCAGAACACTGTATCAACCAGATGCCG
TGTAGTGTCTCTGGGTGAACCGGTTGTAACGTGGCGACGTGCGTGGCAGACGGTCCGTAC
CACCGACCTCGGTGAACTAGGCGCTGGTCAGAACATGCGCGTACGCGTTCATGCCGTGAA
TGGTTACAACTTCGAAGACTCCATACCTCGTATCCGAGCGTGTTGTTCAGGTAAGACCGT
TTCACCACCATCACATTCAGGAACTAGGCGTGTGTGTCGTGACACCAGCTGGGTCCGGAA
GAGATCACCGCGTGACATCGAACGTGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGT
ATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCG
AAAGGTGAAACTCGCATGACACGCAACAGGGGATAGGGACACGCACGCAACAGATGG
>ECTF303_ATG
ATGCGGTGTTATCCGACATTCACGGTCCTGGCCGATTGTACTGCAGGGTGATGACTCTCC
CTGCCGTAACCGTTACGTCTATCGCACTGAACTCGAGCGCGACTACTGTAGGTGCGGTTG
TTATAGGGTCCGTACGCTGACCGTTGCCGTAAGGCATGAGTTAAGTGTACTGGCCGTATC
TGGAAGTTCCGGTTAGGCCGTGGCCGTGCTGGGCCGTGTGGTTAACACTACTGTGACACC
AATCGACGTAGGTCCGCGTGGATCACGACGGCTTCTACTGCTGTAGAAGTCAATCGCTCC
GCGTTATCGAACGTCTAGTCCGTAGATACAGCCGGTACAGACCGGTTATAAAGTCCGTTG
ACTCCATGACTCCCAATCGGTCGTGGTCAGACGTGAATTGATCATCGGTGACGTCAGACA
GGTACCGCACTGGCTATCGATCGCCATCATCAACCAGCGCGATTCCGTATCAAATGTATC
TATGTCGTCTATCGGCCAGAGCGTCCGACCATTTCTAACGTGGTACGTAAACTGAGAGCA
CGGCGCACTAGGCTAACACCATACGTTGTGGTAGCAACCGCGTCGTGAATCCGCGTGCAC
TGTCAATACCTGGCACCGTATAGCCGGTTGCGCAATGGGACGAATACTTCCGTGACCGCG
GTGAAGATGACGCTGATCATTTACGACTGACCTGTCTACAGGCGTGTTGCTTACCGTCGA
GATCTCCCTGCTGCTCCGTACGTCCGCCAGGACGTGAAGCATTACGCGACGTCTACCTCC
ACTCTCGTCTGCTGGAGCGTGCTGCACGTGTTAACGCCGAATACGTTGAAGCCTTCACCA
AAGGTGAAGTGAAAGGGACCGGTTCTCTGACCGCACTGCCGATTATCGAAACTCAGGCGG
GTGACGCATGACACGCAACAGGGGATAGGGACACGCACGCAACAGATGG
>ECTF304_ATG
ATGCCGAAGCAATCCTAGCACGCGACGCGGCGCAGGTACCGGTAGGTGGTTGCAGTGACA
AGATCGATACCAGAAGCTAGATCCGGATCGCGTAAGAACGAACTCTCCCACGTACGGCAT
CCTGCCGGAAGTAGTGCGGTAGAAAGCCGAGTTCGTACACGTATCTGCGAAAGCGTACCG
TATCGATGTAACTGCTGGACGCTATACCTGCTGCACGGCGGAAGTTCGTGGAGCTCGAGC
GGTACGTACGGTATGGCGAGCGGTGACGGTTATCGAATCCTTCCTCGATAGGTCGTGGTC
CGTTGCTACCGTTCTAGGTACGTGAAGGTACTACTGCACAAGGGCGATATCGTTACTGTG
TGGCGTTCGAATACGGTCGTGTTCGTGCGATAGCGTAACGAACTGTCAGGAAGTGCTGGA
AGACGTCCGTCCATTCCGGTGGAAATCCTCGGCCTGTCCGCGTACCGGCTGCGGGTGATC
GAAGTTACCGTTGTACGTGACGAGAAGAGCGCGTAGAAGTTGCACTCTATCGTACAGTAA
ATTCCGCGAAGTTACTAGGCGCGTCAGCGAGATCTACTCGTAGAACATGTTCGCCAACAT
AGACCGAAGGACGAAGTTCACGAAGTAGAATATCGTCCTGAAGGACAGACGTACAGGGTT
CTGTCGAAGACGATCTCCGACTCCTTGCTGTACTGTCTACTGACGAAGTTAAACGTGAAG
ATCATCGGTTCTAGGCGTAGGTGGTATCACCGACGACGCCACTGGCTGACGGCGTCCAAC
GCCATCCTGGTTGGTCTAACGTACGTGCTGATGCCTCTGCACGTAAAGTGATTGAAGCGG
AAAGCCTGGATCTGCGTTACTACTCCGTCATCTATAACCTGATTGACGAAGTGAAAGCGC
ATGACACGCAACAGGGGATAGGGACACGCACGCAACAGATGG
>AVTF2_CAT
CATGCAAGCGTACGCACGTGGTTGTTAAAGCTTTTTTGAAAGTTAATCTCCTGGTTCACC
GTCTGCTCGTATGCGGTTACCAGGTCGGCGGCCGCCACGTGTGCGCGCGCGGGACTAATC
CCGGTTCGCGCGTCGGGCTCAAAGTCCTCCTCGCGCAGCAACCGCTCGCGATTCAGGCCA
TGCCGCAGCTCGCGCCCTGCGTGGAACTTTCGATCCCGCATCTCCTCGGGCTCCTCTCCC
TCGCGGTCGCGAAACAGGTTCTGCCGCGGCACGTACGCCTCACGCGTATCACGCTTCAGC
TGCACCCTTGGGTGCCGCTCAGGAGAGGGCGCTCCTAGCCGCGCCAGGCCCTCGCCCTCC
TCCAAGTCCAGGTAGTGCCGGGCCCGGCGCCGCGGGGGTTCGTAATCACCATCTGCTGCC
GCGTCAACCGCGGATGTCGCCCCTCCTGACGCGGTAGGAGGAGGGGAGGGTGCCCTGCAT
GTCTGCCGCTGCTCTTGCTCTTGCCATG
>AVTF7_CAT
CATGTACCTCTCCGCGTAGGCGCTCGTTGGTCCAGCAGAGGCGGCCGCCCTTGCGCGAGC
AGAATGGCGGTAGGGGGTCTAGCTGCGTCTCGTCCGGGGGGTCTGCGTCCACGGTAAAGA
CCCCGGGCAGCAGGCGCGCGTCGAAGTAGTCTATCTTGCATCCTTGCAAGTCTAGCGCCT
GCTGCCATGCGCGGGCGGCAAGCGCGCGCTCGTATGGGTTGAGTGGGGGACCCCATGGCA
TGGGGTGGGTGAGCGCGGAGGCGTACATGCCGCAAATGTCGTAAACGTAGAGGGGCTCTC
TGAGTATTCCAAGATATGTAGGGTAGCATCTTCCACCGCGGATGCTGGCGCGCACGTAAT
CGTATAGTTCGTGCGAGGGAGCGAGGAGGTCGGGACCGAGGTTGCTACGGGCGGGCTGCT
CTGCTCGGAAGACTATCTGCCTGAAGATGGCATGTGAGTTGGATGATATGGTTGGACGCT
GGAAGACGTTGAAGCTGGCGTCTGTGAGACCTACCGCGTCCATG
>AVTF90_CAT
CATGCGCAAAAACGCAAAACGCAAACGCAACGCACCAGCCTATGCGCCTGGTCTGTACAC
CGTTCATCTGTCCTCTTTCAAAGTTGGTCAGTTCGGTTCCCTTATGATTGACCGTCGGAC
GAGCCCCTTTTTTGCTTTTCCCAGATGCATCCGGTGCTGCGGCAGATGCGCCCCCCTCCT
CAGCAGCGGCAAGAGCAAGAGCAGCGGCAGACATGCAGGGCACCCTCCCCTCCTCCTACC
GCGTCAGGAGGGGCGACATCCGCGGTTGACGCGGCAGCAGATGGTGATTACGAACCCCCG
CGGCGCCGGGCCCGGCACTACCTGGACTTGGAGGAGGGCGAGGGCCTGGCGCGGCTAGGA
GCGCCCTCTCCTGAGCGGCACCCAAGGGTGCAGCTGAAGCGTGATACGCGTGAGGCGTAC
GTGCCGCGGCAGAACCTGTTTCGCGACCGCGAGGGAGAGGAGCCCGAGGAGATGCGGGAT
CGAAAGTTCCACGCAGGGCGCGAGCTGCGGCATGGCCTGAATCGCGAGCGGTTGCTCATG
>AVTF100_CAT
CATGATCCTATCCCTATCCCCTATCCCCCTATTCTCTTGAGGAGTCTCAGCCTCTTAATA
CTTTCATGTTTCAGAATAATAGGTTCCGAAATAGGCAGGGGGCATTAACTGTTTATACGG
GCACTGTTACTCAAGGCACTGACCCCGTTAAAACTTTCATCAGTAACCCGTATCGTGAGC
ATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAG
GCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAG
ACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGT
GAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGAT
GACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCG
GATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGC
GCAGCCATGACCCAGTCATG
>AVTF120_CAT
CATGAGGATAGGGATAGGGGATAGGGGGATAGGCTTAACTCAATTCTTGTGGGTTATCTC
TCTGATATTAGCGCTCAATTACCCTCTGACTTTGTTCAGGGTGTTCAGTTAATTCTCCCG
TCTAATGCGCTTCCCTGTTTTTATGTTATTCTCTCTGTAAAGGCTGCTATTTTCATCTTC
TGCGCTAAGATTGTCAGTTTCCAAAAACGAGGAGGATTTGATATTCACCTGGCCCGCGGT
GATGCCTTTGAGGGTGGCCGCATCCATCTGGTCAGAAAAGACAATCTTTTTGTTGTCAAG
CTTGGTGGCAAACGACCCGTAGAGGGCGTTGGACAGCAACTTGGCGATGGAGCGCAGGGT
TTGGTTTTTGTCGCGATCGGCGCGCTCCTTGGCCGCGATGTTTAGCTGCACGTATTCGCG
CGCAACGCACCGCCATTCGGGAAAGACGGTGGTGCGCTCGTCGGGCACCAGGTGCACGCG
CCAACCGCGGTTGTGCAGGGTGACAAGGTCAACGCTGGTGGCTACCTCTCCGCGTAGGCG
CTCGTTGGTCCAGCAGAGGCGGCCGCCCTTGCGCGACATG
>AVTF150_CAT
CATGGCGTTTTTGCGTTTTGCGTTTGCGTTGCGTTATAACCCAACCTAAGCCGGAGGTTA
AAAAGGTAGTCTCTCAGACCTATGATTTTGATAAATTCACTATTGACTCTTCTCAGCGTC
TTAATCTAAGCTATCGCTATGTTTTCAAGGATTCTAAGGGAAAATTAATTAATAGCGACG
ATTTACAGAAGCAAGGTCGCGGCGACACCTTTGCCACACGGGCTGAGGAGAAGCGCGCTG
AGGCCGAAGCAGCGGCCGAAGCTGCCGCCCCCGCTGCGCAACCCGAGGTCGAGAAGCCTC
AGAAGAAACCGGTGATCAAACCCCTGACAGAGGACAGCAAGAAACGCAGTTACAACCTAA
TAAGCAATGACAGCACCTTCACCCAGTACCGCAGCTGGTACCTTGCATACAACTACGGCG
ACCCTCAGACCGGAATCCGCTCATGGACCCTGCTTTGCACTCCTGACGTAACCTGCGGCT
CGGAGCAGGTCTACTGGTCGTTGCCAGACATGATGCAAGACCCCGTGACCTTCCGCTCCA
CGCGCCAGATCAGCAACTTTCCGGTGGTGGGCGCCGAGCTGTTGCCCGTGCACTCCCATG
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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