Tweet
Anyone have the reference sequences for the CAT and ATG control beads? I can get the fna for the sequences generated (and would assume most frequent are the correct ones) but would like to be sure. Thanks.
-
-
Got them; short versions (from flowgram "ideal" sequence) and full length versions from gsReporter --analysisParms.parse. Thanks, Teri Rambo Mueller @ Roche for the latter info.
>ECTF301_ATG
ATGCCTAGTAAACAATGTTCGATCCGGCGAAGTCTGCAAGAATCCAGCGCTGCCGGTTCG
TCGGCGCTGTGCCGTGGAGCTGACCTGATCGACGACTCGTGCGATCGAGTATGGCTCGCC
ACCTAGAATCGCGACCTATCATCAACGAAGGCGACATCAATACGTCGCCGCGTTAAATCG
TCACTTTCTGCGCACGTCAGCATTACCGCACAGTGCTGGAGTCACTTCCGTGCCGGTCTC
TGCTGGTGACTTCGCAGACCGTCCTGACGTGCTAGGTGGCCGCTTCGCCTGGCAGCCGAT
GAACGGCGTAGATCGGTGCCCTCGCTGCTAGACTGGCGGTTACGAAATGGACGCGCGCAT
CTACTGTGCGAACGTGCTCGCTACCGGACCTGCCGGTATATGGTGAACACCAACACCTCG
GCAGACCTCTCTGAGCCTAGCAGAGCTTCAACCTGGAAGTTCCGGTGACGATCACGAACG
TATCGACGAAAGTTCAGGAATACGTTGCTACTACATCTAACGCTGACTGGATCGAATACT
CTGACTGCACTTCTGTAGCGCAGCCGTCGTCTAGTCTCCGCCTGTCGTTCCGTTATCAGC
TGACTGAACTTGACGCGCAGCGGGCACGTATCGTACTGTCCGGAAGGTGACGAACGCGTA
CCGTTAAAGCAGCCGTCTATCTGTGCTGAACGTAGGTATCGCAACTTGACGTACTGCTGG
GTAATCCGGCAGAGATCACCGTGTTGCAGCGTCTCAGGGTGTAGAACTGGGTGCAGGGAT
TGAAATCGTTGATCCAGAAGTGGTTCGCGAAAGCTATGTTGGTCGTCTGGTCGAACTGCG
TAAGAACAAAGGCATGACCGAAACCGTTGCCCGCGAACAGCTGGAAGACAACGTGGTGCT
CGGTACGCGCATGACACGCAACAGGGGATAGGGACACGCACGCAACAGATGG
>ECTF302_ATG
ATGCTCGACCGACGGTGTTGTAACGTGACGATTACACTACCTGTCTGCTATCGAACGAAG
GCACTACGTTATCGCCCAGGCGAACTCCAACTTCGGATGAAGAAGGTCCACTTCGTAGAA
GACCTGGTAACTTCGCCGTAGCAAAGGCGTAATCCTAGCTTGTTCAGCCGCGACCAGGTT
GTACTACATGGACGTACTCCACAGACAGGTGGTATCCGTCGGTGACGTCTGATCCCGTCC
TGGAACACGTATGACGACCAACCGTGCATTGATAGGGTGCGAACATGACAACGTCAGGCC
GTTCCGACTCTGACGCGCTGATAACGCCGCTGGTTGGTACTGTATGGAACGTGCTGTTGA
CCGTTGACTCCGGTGTAACTAGCGGTAGCTAAACGTGGTGGTGTCGTTCGAGTACGTGGA
TGCTTCCCGTATCGTTATCGAGTTAACGAAGACGAGATGTATCCGGGTGAAGTCAGGTAT
CGACATCTACAACTGACCAAATACACGTTCTAACCAGAACACTGTATCAACCAGATGCCG
TGTAGTGTCTCTGGGTGAACCGGTTGTAACGTGGCGACGTGCGTGGCAGACGGTCCGTAC
CACCGACCTCGGTGAACTAGGCGCTGGTCAGAACATGCGCGTACGCGTTCATGCCGTGAA
TGGTTACAACTTCGAAGACTCCATACCTCGTATCCGAGCGTGTTGTTCAGGTAAGACCGT
TTCACCACCATCACATTCAGGAACTAGGCGTGTGTGTCGTGACACCAGCTGGGTCCGGAA
GAGATCACCGCGTGACATCGAACGTGTGAAGCTGCGCTCTCCAAACTGGATGAATCCGGT
ATCGTTTACATTGGTGCGGAAGTGACCGGTGGCGACATTCTGGTTGGTAAGGTAACGCCG
AAAGGTGAAACTCGCATGACACGCAACAGGGGATAGGGACACGCACGCAACAGATGG
>ECTF303_ATG
ATGCGGTGTTATCCGACATTCACGGTCCTGGCCGATTGTACTGCAGGGTGATGACTCTCC
CTGCCGTAACCGTTACGTCTATCGCACTGAACTCGAGCGCGACTACTGTAGGTGCGGTTG
TTATAGGGTCCGTACGCTGACCGTTGCCGTAAGGCATGAGTTAAGTGTACTGGCCGTATC
TGGAAGTTCCGGTTAGGCCGTGGCCGTGCTGGGCCGTGTGGTTAACACTACTGTGACACC
AATCGACGTAGGTCCGCGTGGATCACGACGGCTTCTACTGCTGTAGAAGTCAATCGCTCC
GCGTTATCGAACGTCTAGTCCGTAGATACAGCCGGTACAGACCGGTTATAAAGTCCGTTG
ACTCCATGACTCCCAATCGGTCGTGGTCAGACGTGAATTGATCATCGGTGACGTCAGACA
GGTACCGCACTGGCTATCGATCGCCATCATCAACCAGCGCGATTCCGTATCAAATGTATC
TATGTCGTCTATCGGCCAGAGCGTCCGACCATTTCTAACGTGGTACGTAAACTGAGAGCA
CGGCGCACTAGGCTAACACCATACGTTGTGGTAGCAACCGCGTCGTGAATCCGCGTGCAC
TGTCAATACCTGGCACCGTATAGCCGGTTGCGCAATGGGACGAATACTTCCGTGACCGCG
GTGAAGATGACGCTGATCATTTACGACTGACCTGTCTACAGGCGTGTTGCTTACCGTCGA
GATCTCCCTGCTGCTCCGTACGTCCGCCAGGACGTGAAGCATTACGCGACGTCTACCTCC
ACTCTCGTCTGCTGGAGCGTGCTGCACGTGTTAACGCCGAATACGTTGAAGCCTTCACCA
AAGGTGAAGTGAAAGGGACCGGTTCTCTGACCGCACTGCCGATTATCGAAACTCAGGCGG
GTGACGCATGACACGCAACAGGGGATAGGGACACGCACGCAACAGATGG
>ECTF304_ATG
ATGCCGAAGCAATCCTAGCACGCGACGCGGCGCAGGTACCGGTAGGTGGTTGCAGTGACA
AGATCGATACCAGAAGCTAGATCCGGATCGCGTAAGAACGAACTCTCCCACGTACGGCAT
CCTGCCGGAAGTAGTGCGGTAGAAAGCCGAGTTCGTACACGTATCTGCGAAAGCGTACCG
TATCGATGTAACTGCTGGACGCTATACCTGCTGCACGGCGGAAGTTCGTGGAGCTCGAGC
GGTACGTACGGTATGGCGAGCGGTGACGGTTATCGAATCCTTCCTCGATAGGTCGTGGTC
CGTTGCTACCGTTCTAGGTACGTGAAGGTACTACTGCACAAGGGCGATATCGTTACTGTG
TGGCGTTCGAATACGGTCGTGTTCGTGCGATAGCGTAACGAACTGTCAGGAAGTGCTGGA
AGACGTCCGTCCATTCCGGTGGAAATCCTCGGCCTGTCCGCGTACCGGCTGCGGGTGATC
GAAGTTACCGTTGTACGTGACGAGAAGAGCGCGTAGAAGTTGCACTCTATCGTACAGTAA
ATTCCGCGAAGTTACTAGGCGCGTCAGCGAGATCTACTCGTAGAACATGTTCGCCAACAT
AGACCGAAGGACGAAGTTCACGAAGTAGAATATCGTCCTGAAGGACAGACGTACAGGGTT
CTGTCGAAGACGATCTCCGACTCCTTGCTGTACTGTCTACTGACGAAGTTAAACGTGAAG
ATCATCGGTTCTAGGCGTAGGTGGTATCACCGACGACGCCACTGGCTGACGGCGTCCAAC
GCCATCCTGGTTGGTCTAACGTACGTGCTGATGCCTCTGCACGTAAAGTGATTGAAGCGG
AAAGCCTGGATCTGCGTTACTACTCCGTCATCTATAACCTGATTGACGAAGTGAAAGCGC
ATGACACGCAACAGGGGATAGGGACACGCACGCAACAGATGG
>AVTF2_CAT
CATGCAAGCGTACGCACGTGGTTGTTAAAGCTTTTTTGAAAGTTAATCTCCTGGTTCACC
GTCTGCTCGTATGCGGTTACCAGGTCGGCGGCCGCCACGTGTGCGCGCGCGGGACTAATC
CCGGTTCGCGCGTCGGGCTCAAAGTCCTCCTCGCGCAGCAACCGCTCGCGATTCAGGCCA
TGCCGCAGCTCGCGCCCTGCGTGGAACTTTCGATCCCGCATCTCCTCGGGCTCCTCTCCC
TCGCGGTCGCGAAACAGGTTCTGCCGCGGCACGTACGCCTCACGCGTATCACGCTTCAGC
TGCACCCTTGGGTGCCGCTCAGGAGAGGGCGCTCCTAGCCGCGCCAGGCCCTCGCCCTCC
TCCAAGTCCAGGTAGTGCCGGGCCCGGCGCCGCGGGGGTTCGTAATCACCATCTGCTGCC
GCGTCAACCGCGGATGTCGCCCCTCCTGACGCGGTAGGAGGAGGGGAGGGTGCCCTGCAT
GTCTGCCGCTGCTCTTGCTCTTGCCATG
>AVTF7_CAT
CATGTACCTCTCCGCGTAGGCGCTCGTTGGTCCAGCAGAGGCGGCCGCCCTTGCGCGAGC
AGAATGGCGGTAGGGGGTCTAGCTGCGTCTCGTCCGGGGGGTCTGCGTCCACGGTAAAGA
CCCCGGGCAGCAGGCGCGCGTCGAAGTAGTCTATCTTGCATCCTTGCAAGTCTAGCGCCT
GCTGCCATGCGCGGGCGGCAAGCGCGCGCTCGTATGGGTTGAGTGGGGGACCCCATGGCA
TGGGGTGGGTGAGCGCGGAGGCGTACATGCCGCAAATGTCGTAAACGTAGAGGGGCTCTC
TGAGTATTCCAAGATATGTAGGGTAGCATCTTCCACCGCGGATGCTGGCGCGCACGTAAT
CGTATAGTTCGTGCGAGGGAGCGAGGAGGTCGGGACCGAGGTTGCTACGGGCGGGCTGCT
CTGCTCGGAAGACTATCTGCCTGAAGATGGCATGTGAGTTGGATGATATGGTTGGACGCT
GGAAGACGTTGAAGCTGGCGTCTGTGAGACCTACCGCGTCCATG
>AVTF90_CAT
CATGCGCAAAAACGCAAAACGCAAACGCAACGCACCAGCCTATGCGCCTGGTCTGTACAC
CGTTCATCTGTCCTCTTTCAAAGTTGGTCAGTTCGGTTCCCTTATGATTGACCGTCGGAC
GAGCCCCTTTTTTGCTTTTCCCAGATGCATCCGGTGCTGCGGCAGATGCGCCCCCCTCCT
CAGCAGCGGCAAGAGCAAGAGCAGCGGCAGACATGCAGGGCACCCTCCCCTCCTCCTACC
GCGTCAGGAGGGGCGACATCCGCGGTTGACGCGGCAGCAGATGGTGATTACGAACCCCCG
CGGCGCCGGGCCCGGCACTACCTGGACTTGGAGGAGGGCGAGGGCCTGGCGCGGCTAGGA
GCGCCCTCTCCTGAGCGGCACCCAAGGGTGCAGCTGAAGCGTGATACGCGTGAGGCGTAC
GTGCCGCGGCAGAACCTGTTTCGCGACCGCGAGGGAGAGGAGCCCGAGGAGATGCGGGAT
CGAAAGTTCCACGCAGGGCGCGAGCTGCGGCATGGCCTGAATCGCGAGCGGTTGCTCATG
>AVTF100_CAT
CATGATCCTATCCCTATCCCCTATCCCCCTATTCTCTTGAGGAGTCTCAGCCTCTTAATA
CTTTCATGTTTCAGAATAATAGGTTCCGAAATAGGCAGGGGGCATTAACTGTTTATACGG
GCACTGTTACTCAAGGCACTGACCCCGTTAAAACTTTCATCAGTAACCCGTATCGTGAGC
ATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAG
GCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAG
ACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGT
GAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGAT
GACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCG
GATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGC
GCAGCCATGACCCAGTCATG
>AVTF120_CAT
CATGAGGATAGGGATAGGGGATAGGGGGATAGGCTTAACTCAATTCTTGTGGGTTATCTC
TCTGATATTAGCGCTCAATTACCCTCTGACTTTGTTCAGGGTGTTCAGTTAATTCTCCCG
TCTAATGCGCTTCCCTGTTTTTATGTTATTCTCTCTGTAAAGGCTGCTATTTTCATCTTC
TGCGCTAAGATTGTCAGTTTCCAAAAACGAGGAGGATTTGATATTCACCTGGCCCGCGGT
GATGCCTTTGAGGGTGGCCGCATCCATCTGGTCAGAAAAGACAATCTTTTTGTTGTCAAG
CTTGGTGGCAAACGACCCGTAGAGGGCGTTGGACAGCAACTTGGCGATGGAGCGCAGGGT
TTGGTTTTTGTCGCGATCGGCGCGCTCCTTGGCCGCGATGTTTAGCTGCACGTATTCGCG
CGCAACGCACCGCCATTCGGGAAAGACGGTGGTGCGCTCGTCGGGCACCAGGTGCACGCG
CCAACCGCGGTTGTGCAGGGTGACAAGGTCAACGCTGGTGGCTACCTCTCCGCGTAGGCG
CTCGTTGGTCCAGCAGAGGCGGCCGCCCTTGCGCGACATG
>AVTF150_CAT
CATGGCGTTTTTGCGTTTTGCGTTTGCGTTGCGTTATAACCCAACCTAAGCCGGAGGTTA
AAAAGGTAGTCTCTCAGACCTATGATTTTGATAAATTCACTATTGACTCTTCTCAGCGTC
TTAATCTAAGCTATCGCTATGTTTTCAAGGATTCTAAGGGAAAATTAATTAATAGCGACG
ATTTACAGAAGCAAGGTCGCGGCGACACCTTTGCCACACGGGCTGAGGAGAAGCGCGCTG
AGGCCGAAGCAGCGGCCGAAGCTGCCGCCCCCGCTGCGCAACCCGAGGTCGAGAAGCCTC
AGAAGAAACCGGTGATCAAACCCCTGACAGAGGACAGCAAGAAACGCAGTTACAACCTAA
TAAGCAATGACAGCACCTTCACCCAGTACCGCAGCTGGTACCTTGCATACAACTACGGCG
ACCCTCAGACCGGAATCCGCTCATGGACCCTGCTTTGCACTCCTGACGTAACCTGCGGCT
CGGAGCAGGTCTACTGGTCGTTGCCAGACATGATGCAAGACCCCGTGACCTTCCGCTCCA
CGCGCCAGATCAGCAACTTTCCGGTGGTGGGCGCCGAGCTGTTGCCCGTGCACTCCCATG
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment