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  • animesh
    Junior Member
    • Feb 2008
    • 1

    Getting Newbler

    Is there any demo copy available for Newbler?
  • zgyzsl
    Junior Member
    • Sep 2008
    • 1

    #2
    yes, I am interested in this assembler too.who can give us a reply.

    Comment

    • bioinfosm
      Senior Member
      • Jan 2008
      • 483

      #3
      I think you only get it if you have a 454... (with all the licensing, etc)
      --
      bioinfosm

      Comment

      • Layla
        Member
        • Sep 2008
        • 58

        #4
        yes this is true. If you have the 454 software with the licence then you can use it

        Comment

        • dan
          wiki wiki
          • Jul 2008
          • 194

          #5
          What is the alternative?
          Homepage: Dan Bolser
          MetaBase the database of biological databases.

          Comment

          • Layla
            Member
            • Sep 2008
            • 58

            #6
            There are other commercial software available to analyse your 454 without using gsMapper/DeNovo or gsAssembler. The only one I can currently remember is Biogene

            Comment

            • dan
              wiki wiki
              • Jul 2008
              • 194

              #7
              I was specifically thinking of non commercial tools. I think I'll try the wgs-assembler (Celera assembler).

              Cheers,
              Homepage: Dan Bolser
              MetaBase the database of biological databases.

              Comment

              • sklages
                Senior Member
                • May 2008
                • 628

                #8
                wgs-assembler makes a good job on sanger, FLX and Titanium data either alone
                or as hybrid assemblies ..

                It is not working with short read data (illiumina, solid) and is not optimised for
                old gs20 data.

                cheers,
                Sven

                Comment

                • strob
                  Member
                  • Nov 2008
                  • 84

                  #9
                  If you use 454 data you can just ask for the Newbler software. We did it this way and we received a DVD containing everything we need.

                  Comment

                  • v_kisand
                    Member
                    • Jan 2009
                    • 37

                    #10
                    MIRA should be nice alternative as well. I would go on wgs or MIRA ... or check velvet... in my mind these three are the most promising. I tested a bit CLC Genome Workbench and was not particularly impressed...

                    Originally posted by strob View Post
                    If you use 454 data you can just ask for the Newbler software. We did it this way and we received a DVD containing everything we need.

                    Comment

                    • sram
                      Junior Member
                      • Jun 2009
                      • 7

                      #11
                      Not so here in Chile...

                      Originally posted by strob View Post
                      If you use 454 data you can just ask for the Newbler software. We did it this way and we received a DVD containing everything we need.
                      I did that and I was told I can get the software only if I have a GS FLX system. I get this answer from a representant of Roche here in Chile.

                      ---sram

                      Comment

                      • dan
                        wiki wiki
                        • Jul 2008
                        • 194

                        #12
                        I really have no idea why Roche insist that only people with the sequencer are allowed the software... They are actively damaging their software's status among the bioinformatics community.

                        Seriously, what do they expect? Only sequencing centres will do bioinf analysis with 454 data? Clearly not. Therefore we are forced by Roche NOT to use Roche products... What kind of crazy logic is that?

                        Dear Roche,

                        Please make Newbler open source immediately.

                        Sincerely,

                        Someone who imagines that you actually care about improving your software and increasing its user base.


                        sram, try writing to the people who gave you the data, and ask them to ask Roach on your behalf.
                        Homepage: Dan Bolser
                        MetaBase the database of biological databases.

                        Comment

                        • strob
                          Member
                          • Nov 2008
                          • 84

                          #13
                          Again, I had no trouble getting the software. They just asked me where we get our sequences from (which provider), and a few days later the DVD's were in my mailbox....

                          Comment

                          • sram
                            Junior Member
                            • Jun 2009
                            • 7

                            #14
                            Originally posted by dan View Post
                            I really have no idea why Roche insist that only people with the sequencer are allowed the software... They are actively damaging their software's status among the bioinformatics community.

                            Seriously, what do they expect? Only sequencing centres will do bioinf analysis with 454 data? Clearly not. Therefore we are forced by Roche NOT to use Roche products... What kind of crazy logic is that?

                            Dear Roche,

                            Please make Newbler open source immediately.

                            Sincerely,

                            Someone who imagines that you actually care about improving your software and increasing its user base.


                            sram, try writing to the people who gave you the data, and ask them to ask Roach on your behalf.
                            Dan, thank you. I will do that. I am receiving the data from research centres in Europe who work with us in some projects. In fact some samples are ours and they are supporting us with the sequencing. Perhaps Roche have a different policy in Europe.. taking in account the message from strob, who appear to be writing from Belgium.
                            I totally agree with you about Roche should make this software available for academic research. For commercial uses is another thing.

                            ---sram

                            Comment

                            • strob
                              Member
                              • Nov 2008
                              • 84

                              #15
                              I work indeed in Belgium, Europe. And I even work in a commercial company....

                              At the PAG meeting I discussed this with Roche at their booth and they just told me that I should contact my local contact. After 1 phone cal, I received the software.

                              Comment

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