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Anybody have an idea what the emPCR additive actually is?
DMSO? Betaine? Tw20? Magic?
DMSO? Betaine? Tw20? Magic?
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Must be some magic, because some replaced that with DMSO and betaine (like Illumina does) and have much better yields during emulsion breaking. -
not sure what it is exactly, a stabilizer i presume. anyway, tech support has told me that not spinning down the undissolved precipitate in the additive and adding the precipitate to our emulsion can cause the emulsions to break. They said just get most of the supernatant even though it is less than the required amount and it should be fine.Comment
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It's trehalose...Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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