Anybody have an idea what the emPCR additive actually is?
DMSO? Betaine? Tw20? Magic?
DMSO? Betaine? Tw20? Magic?
-
-
Must be some magic, because some replaced that with DMSO and betaine (like Illumina does) and have much better yields during emulsion breaking. -
not sure what it is exactly, a stabilizer i presume. anyway, tech support has told me that not spinning down the undissolved precipitate in the additive and adding the precipitate to our emulsion can cause the emulsions to break. They said just get most of the supernatant even though it is less than the required amount and it should be fine.Comment
-
It's trehalose...Comment
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment