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  • a Great 454 Run

    Hi all,

    I've made a great run, i will share my mods when then output is validated.






    the throughput was more than 700MB and it's the regular FLX.

  • #2
    Awesome! Congrats!

    Comment


    • #3
      Yes, this is how far you can stretch GS FLX Titanium (we have seen the same on our instrument). Congrats!

      Comment


      • #4
        Nicely done!

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        • #5
          These are amazing numbers! This is a shotgun library, presumably. What was your DNA:bead ratio and how did you determine that ratio?

          Thanks.

          Barry

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          • #6
            well I've targeted a 14% enrichment just to overcome the two oil cups and run the full ptp using one cup emulsion and everything was chilled. the library size or the fragmentation was from 900-2.5kb the majority was around 1.1-1.2kb.

            yet still to validate, another run was performed with the same modifications and the throughput was 700+ too.

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            • #7
              @mohdsos - excellent achievement!

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