a Great 454 Run

[mohdsos]

Hi all,

I've made a great run, i will share my mods when then output is validated.






the throughput was more than 700MB and it's the regular FLX.

[zhengz]

Awesome! Congrats!

[flxlex]

Yes, this is how far you can stretch GS FLX Titanium (we have seen the same on our instrument). Congrats!

[Anthony.287]

Nicely done!

[Dynamac]

These are amazing numbers! This is a shotgun library, presumably. What was your DNA:bead ratio and how did you determine that ratio?

Thanks.

Barry

[mohdsos]

well I've targeted a 14% enrichment just to overcome the two oil cups and run the full ptp using one cup emulsion and everything was chilled. the library size or the fragmentation was from 900-2.5kb the majority was around 1.1-1.2kb.

yet still to validate, another run was performed with the same modifications and the throughput was 700+ too.

[hengnck]

@mohdsos - excellent achievement!